(2010) Validation of a quantitative flow cytometer assay for monitoring HER-2/neu expression level in cell-based cancer immunotherapy products. Randlev B, Huang L-C, Watatsu M, Marcus M, Lin A, Shih S-J. Optimization of receptor occupancy assays in mass cytometry: Standardization across channels with QSC beads. H., Bader, L., Blaser, N., Budzinski, L., Schulz, A.
PDS 814A, QSC/SC Antibody Titration Protocolīringeland, G. Quantum™ Simply Cellular ® kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests). For additional information about working with these products, see our product data sheet on Antibody Titration or check out PDS 818, Tips and Troubleshooting.
QUANTUM CALCULATOR YOUTUBE FREE
A free QuickCal ® analysis template is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold. The investigator labels QSC beads using the same antibodies used to stain cells. Each coated population binds a specific number of monoclonal antibodies of the noted species, which is equal to its Antibody Binding Capacity (ABC) value. Quantum™ Simply Cellular® (QSC) kits are comprised of five microsphere populations that are ~7-9µm in diameter: one blank and four labeled with increasing amounts of antibody. (Quantum MESF to confirm and quantitate transfection efficiency featuring a fluorescently-labeled vector) Quantum™ Simply Cellular® (2017) A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells. Homann S, Hofmann C, Gorin AM, Nguyen HCX, Huynh D, Hamid P. (2013) Manufacture of Clinical-Grade CD19-Specific T Cells Stably Expressing Chimeric Antigen Receptor Using Sleeping Beauty System and Artificial Antigen Presenting Cells.PLoS ONE 8(5):e64138 Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, et al. Muller L, Mitsuhashi M, Simms P, Gooding WE, Whiteside TL (2016) Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets. (2006) Quantitative flow cytometry of ZAP-70 levels in chronic lymphocytic leukemia using molecules of equivalent soluble fluorochrome. Kay S, Herishanu Y, Pick M, Rogowski, O, Baron S, Naparstek E, Polliack A, Deutsch, V. Smith ML, Chyla B, McKeegan E, Tahir SK (2016) Development of a flow cytometric method for quantification of BCL-2 family members in chronic lymphocytic leukemia and correlation with sensitivity to BCL-2 family inhibitors. (2013) Lymphocyte crossmatching by flow cytometry. Cytometry B Clin Cytom 90B:177-190.īray RA. (2016) Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug development. Quadrini KJ, Hegelund AC, Cortes KE, Xue C, Kennelly SM, Ji H, Högerkorp C-M, Mc Closky TW. (2008) Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor agonist. SDS ST334, (QMESF AF 488, PE & APC) Catalog Number
PDS 849, Determination of Effective F/P Ratio Quantum™ MESF kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests). See PDS 849 to determine effective FP ratio. The assignment of fluorescence intensity in Molecules of Soluble Fluorochrome (MESF) units is performed through direct comparison of fluorescence measurements from solutions of the pure fluorochrome with those from microspheres surface-labeled with the same fluorochrome.
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Our Quantum™ MESF kits contain one blank population and a series of either four fluorescent microsphere populations labeled with varying amounts of Alexa Fluor ® 488, Alexa Fluor ® 647, APC, or R-PE, or five fluorescent microsphere populations labeled with varying amounts of FITC. quantitation of the fluor signal from each cell population).įor additional information about working with these products, see our Quantitative Cytometry brochure and our Tips & Troubleshooting PDS or check out our Flow Blog. Cell samples are then read against the curve for determination of expression (i.e. To use, beads are run on the same day and at the same PMT & compensation settings as samples to establish a calibration curve that relates instrument channel values and standardized fluorescence intensity units (MESF or ABC). Because they are labeled with the same fluorochromes used to label cells, they provide a synchronous response to the environment. Our Quantum™ MESF and Quantum™ Simply Cellular ® beads are external standards that enable the standardization of fluorescence intensity units irrespective of cytometer and software.
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